![]() ![]() Some commands are lengthy and if users need to apply different parameters, they must repeat these lengthy command lines many times. ![]() In QIIME2 and Mothur, for example, the command line is the main user interface, which requires considerable keyboard work. Although these bioinformatics tools can process NGS data and assist in discovery of underlying mechanisms, most are executed in the Linux operating system, which requires system knowledge to handle the software installation and familiarity with the use of command line interface. Accordingly, several 16S bioinformatics tools have been developed, such as Quantitative Insights Into Microbial Ecology 2 (QIIME2) and Mothur. To understand the composition of the microbiome in a particular environment, the genetic marker in prokaryotes, 16S ribosomal DNA (rDNA), which encodes 16S ribosomal RNA (rRNA) has often been sequenced. Since the advent of next-generation sequencing (NGS) technology and the initiation of the Human Microbiome Project, research into microbial composition related to human disease, environment, and diet has been increasingly pursued. The web server and documentation are freely available at. EasyMAP is a user-friendly tool for comprehensive analysis of 16S ribosomal DNA sequencing data. Users can perform quality checks, taxonomy differential abundance analysis, microbial gene function prediction and longitudinal analysis with step-by-step guidance. EasyMAP integrates the QIIME2, LefSe, and PICRUSt pipelines and includes temporal profiling analysis. Here, a comprehensive and easy-to-use online platform, Easy Microbiome Analysis Platform (EasyMAP), has been developed for analysis of 16S ribosomal DNA sequencing data. Sequencing of 16S ribosomal DNA allows the composition of bacteria and archaea in a sample to be obtained and many analytical tools related to 16S ribosomal DNA sequences have been proposed however, most do not include a user-friendly platform with a graphical user interface. As next-generation sequencing technology has become more advanced, research on microbial 16S ribosomal DNA sequences has developed rapidly. ![]()
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